DOT (Department of Transportation) urine collection is used for drug and alcohol testing purposes, and is required for employees in safety-sensitive positions as mandated by federal regulations in the United States. Businesses and organizations that fall under the purview of these regulations are required to collect urine samples for testing from employees in safety-sensitive positions. This includes transportation companies such as airlines, trucking and bus companies, as well as companies that employ commercial drivers. Other organizations that may require DOT urine collection include government agencies, law enforcement agencies, and sports organizations. The purpose of DOT urine collection is to ensure that individuals in safety-sensitive positions are not under the influence of drugs or alcohol while performing their duties, thus promoting safety and minimizing the risk of accidents or errors.